Simplicity 4000 User Manual

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Universal
Illumination
System
All wavelengths included
Simply Better Control
pE-4000
Powerful
Ecient
Compact
www.CoolLED.com
Simply Better Control
Manual: Dual function remote manual control pod for White mode or Advanced mode
Remote: Via USB for independent on/o and intensity control of each channel. Triggering speed <1ms
Via 4 TTL inputs for independent on/o control of each channel. Triggering speed <20us
Via single TTL for on/o control of manual or software selected channels
Via 4 analogue inputs 0-10V, 0-300kHz for dynamic control of intensity from external analogue signals
Synch Out: 4 TTL outputs for each channel – active high
1 TTL output for any channel – active high
Programmable 4 TTL outputs for on/o control of peripherals (transmitted illuminators, stages etc.).
interface: 4 Analogue outputs for intensity control of peripherals (can be programmed to mirror LED intensities for channel control) 0-10V full scale.)
Function Generator: Internally generated sine, pulse and ramps for each channel programmed via control pod
Connectivity: USB (B type) for PC connection. All other TTL and Analogue inputs/outputs via 25way ‘D-type’ female connector
(optional rear mounting pE-Expansion Box available for BNC connectivity).
Imaging Software: Recognised as a CoolLED pE-2 peripheral under common software e.g. Micromanager, MetaMorph, cellSens, NIS Elements, ImagePro, etc.
Power requirements: 110-240Va.c. 50/60Hz, 2.5A
Power consumption: Standby (i.e. no LEDs on) Max 7W
Single wavelength operation Max 41W
Dual wavelength operation Max 75W
Triple wavelength operation Max 93W
Quad wavelength operation Max 112W
pE-4000 light source: 150mm(w) x 220mm(d) x 260mm(h) Weight 3.5kg
pE-4000 control pod: 154mm(w) x 135mm(d) x 40mm(h) Weight 0.95kg
pE-4000 power supply: 164mm(w) x 64mm(d) x 35mm(h) Weight 0.58kg
pE-Expansion Box: 151mm(w) x 18mm(d) x 95mm(h) Weight 0.34kg
pE-4000-L-SYS-ZZ: pE-4000 Light Source with manual control pod, and power supply for liquid light guide delivery
pE-4000-F-SYS-ZZ: pE-4000 Light Source with manual control pod and power supply for ber delivery
pE-4000-EB25D: Rear mounting pE-Expansion Box for 25-way D-type to BNC connectivity
pE-4000-EFH-4: Excitation lter holders (4 o) to accept 25mm diameter lters
pE-1904: 1m long, 3mm diameter liquid light guide
pE-1908: 3m long, 3mm diameter liquid light guide
pE-10400: Universal Collimator for use with a liquid light guide. Requires additional microscope adaptor
pE-ADAPTOR-YYY: Microscope Adaptor to customer-specied microscope
A range of bers is available from CoolLED, see Accessories (http://www.coolled.com/product-detail/accessories/)
To specify microscope adaptor (YYY), see Adaptors (http://www.coolled.com/product-detail/adaptors-2/)
To specify Power Cable (ZZ): 10 = Australia, 20 = Europe, 30 = UK, 40 = USA
Warranty: System = 12 months, LEDs = 36 months.
pE-4000 illumination system: Light source with complete set of wavelengths, manual control pod, and power supply
Light delivery: Single liquid light guide or ber options
Collimating optics: Universal collimator for use with a single liquid light guide. Requires additional microscope adaptor
LED wavelengths: LED wavelengths are divided across 4 channels with each channel having individual control
Specication
Control & Interface
Power
Dimensions
To Order
Channel 1 Channel 2 Channel 3 Channel 4
Wavelength
(nm)
Power
(mW)
FWHM
(nm)
Wavelength
(nm)
Power
(mW)
FWHM
(nm)
Wavelength
(nm)
Power
(mW)
FWHM
(nm)
Wavelength
(nm)
Power
(mW)
FWHM
(nm)
365 30 10 460 241 20 525 35 31 635 136 16
385 101 10 470 167 20 550 245 85 660 193 19
405 188 18 490 87 31 580 236 85 740 76 39
435 170 17 500 58 29 595 37 15 770 18 53
Powers measured at sample plane of research grade microscope using 100% mirror in cube and 10X objectives. (Figures are
typical values). Note wavelength settings 550nm and 580nm use single broad, high intensity peak (see spectrum on pages 2-3)
Due to a programme of continual development, performance values may change. Correct at time of print.
Environment & Safety
LED products are more sustainable and energy ecient than
conventional illuminators. CoolLED’s products have the
following benets::
• Mercury-free
• Energy Ecient: 80% less power
• Long lifetime (25,000 operating hours)
• No bulb replacements
• Reduced risk of eye damage
• Quiet operation
• No special disposal regulations or issues
For more information on how CoolLED products can help you, contact us now:
t: +44 (0)1264 323040 (Worldwide) 1-800-877-0128 (USA/Canada)
w: www.CoolLED.com
e: info@CoolLED.com
www.CoolLED.com
August 2014
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Summary of Contents

Page 1 - Illumination

Universal Illumination SystemAll wavelengths includedSimply Better ControlpE-4000• Powerful • Ecient• Compactwww.CoolLED.comSimply Better ControlManu

Page 2 - Advanced

pE-4000The pE-4000 sets the standard as the universal illumination system for uorescence microscopy. The system has 16 selectable LED wavelengths tha

Page 3

Universal Illumination SystemAll wavelengths includedSimply Better ControlpE-4000• Powerful • Ecient• Compactwww.CoolLED.comSimply Better ControlManu

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